It therefore represents the state-of-the-art for the detection, measurement and functional analysis of immune cells.
EliSpot Applications in Research and Diagnostics Elispot is now widely used as a method to determine cytokine secretion, especially in vaccine development.
The antigen specific secretion of IFN-gamma also is used for diagnostic procedures. We include some references without any claim to completeness. Replacing the Skin Prick: Tuberculin skin testing and in vitro T cell responses to ESAT-6 and culture filtrate protein 10 after infection with Mycobacterium marinum or M.
Comparison of T-cell-based assay with tuberculin skin test for diagnosis of Mycobacterium tuberculosis infection in a school tuberculosis outbreak. Rapid detection of active and latent tuberculosis infection in HIV-positive individuals by enumeration of Mycobacterium tuberculosis-specific T cells.
Enhanced contact tracing and spatial tracking of Mycobacterium tuberculosis infection by enumeration of antigen-specific T cells. Rapid detection of Mycobacterium tuberculosis infection by enumeration of antigen-specific T cells.
Enumeration of T cells specific for RD1-encoded antigens suggests a high prevalence of latent Mycobacterium tuberculosis infection in healthy urban Indians.
Epub Jan Epub Jun Identification of novel Mycobacterium tuberculosis antigens with potential as diagnostic reagents or subunit vaccine candidates by comparative genomics.
CD8 cytotoxic T cells and the development of new tuberculosis vaccines. Lalvani A, Hill AV. Lyme Disease The Lyme-Disease can trigger an autoimmune reaction. Elispot assay for Chlamydia-specific, antibody-producing cells correlated with conventional complement fixation and microimmunofluorescence.
J Clin Lab Anal IFN gamma and interleukin 4 secreting cells in the gastric antrum in Helicobacter pylori positive and negative gastritis. The response of the T-lymphocytes to stimulating antigens or mitogens is significantly reduced.
Design and validation of an enzyme-linked immunosorbent spot assay for use in clinical trials of candidate HIV vaccines.
Correlates of nontransmission in US women at high risk of human immunodeficiency virus type 1 infection through sexual exposure. A sensitive Elispot assay to detect low-frequency human T-lymphocytes. J Immunol Methods 2: IFN-gamma, produced by Th1-cells, counters this effect. They correlate with the IgE-titers in sera of persons suffering from atopies.
Specific induction of interleukinproducing cells in response to in vitro allergen stimulation in atopic individuals. Clin Exp Allergy Allograft Rejection In recipients of allogenetic renal transplants a quotient of IFN-gamma to Interleukin-5 higher than 15 correlates with an allograft rejection.Applications in diagnosis and research & literature Literature References: EliSpot Applications in Research and Diagnostics Elispot is now widely used as a method to determine cytokine secretion, especially in vaccine development.
ELISpot assays are used in a range of applications to monitor immunological response patterns, and their utility for the identification of antigenic peptides has proven useful for the development of immunotherapeutics and vaccine strategies.
ImmunoSpot® APPLICATIONS ELISPOT Assays The ELISPOT assay is the most sensitive technique available for the detection of immune cells which secrete specific signature proteins such as .
ELISPOT assays The ELISPOT assay is the most sensitive technique available for the detection of immune cells which secrete specific signature proteins such as antibodies or cytokines. It therefore represents the state-of-the-art for the detection, measurement and functional analysis of immune cells.
Antibodies & Buffers. Conjugated antibodies, purified antibodies, and buffers for cell biology and immunology. Cell-Based Assays. Assays, kits, and recombinant proteins for measuring cellular function including apoptosis, cell cycle, and proliferation.
Cell Sample Preparation and Shipping. All cellular analysis services are carried out at ProImmune’s qualified facilities. ProImmune has substantial experience in the shipping of customer cell samples from anywhere in the world.